Kv channel recording solutions

[Kv channel currents]

Bath Solution

NaCl 135 mM 7.889 g /1000 ml ----- 140

KCl 5.4 mM 0.4026 g/1000 ml -------4

CaCl₂ (2H₂O) 1.0 mM 0.147 g/1000 ml --------2

MgCl₂ (6H₂O) 1.2 mM 0.244 g/1000 ml---------1

HEPEs 10 mM 2.383 g/1000 ml ---------10

Glucose 5 mM 0.901 g/1000 ml ----------x

(Adjust pH to 7.3 with NaOH)

Pipette Solution

KCl 140 mM 1.0437 g/100 ml -------- 140

CaCl₂ (2H₂O) 1.0 mM 0.0147 g/100 ml ---------X

MgCl₂ (6H₂O)1.0 mM 0.0203 g/100 ml ---------1

HEPES 10.0 mM 0.2383 g/100 ml ----------10

EGTA 10.0 mM 0.3804 g/100 ml ------------1

(Adjust pH to 7.3 with KOH)