50 X TAE Buffer Preparation protocol (Tris-Acetate-EDTA)

 

242 gm            -           Tris base

57.1 mL           -           Acetic Acid

100mL             -           0.5 M EDTA (shake vigorously before use)

 

Add ddH2O to 1 Liter and adjust ph to 8.5 using KOH.